Pigment or Organism Name:	Bacteriochlorophyll c (Frigaard)
Type:	whole cells
Medium Type:	whole cell
Description:	In vivo absorbance spectrum of BChl c combined with carotenoids of unspecified type and amount


x-axis label:	wavelength
x-axis units:	nm
y-axis label:	absorbance
y-axis units:	relative
error label:	nil
error units:	nil


Source/Contact:	Niels-Ulrik Frigaard

Reference:	Frigaard, N.-U., G. D. Voigt, et al. (2002). "Chlorobium tepidum Mutant Lacking Bacteriochlorophyll c Made by Inactivation of the bchK Gene, Encoding Bacteriochlorophyll c Synthase." Journal of Bacteriology 184(12): 3368-3376.


Comments:
	Absorbance (in vivo), A, of intact cells of Chlorobium tepidum (green sulfur bacteria) containing BChl c, BChl a, and carotenoids, where A(lambda) = -log10(J(lambda)/J0(lambda)), where J0(lambda) is the incident light at wavelength lambda, and J(lambda) is the transmitted or attenuated light. Aqueous buffer.
	The spectrum is a combination of the major pigment BChl c (peaks around 460 and 750 nm) and the minor pigments BChl a (shoulder around 820 nm) and carotenoids (shoulder around 520 nm). 
	From Niels-Ulrik Frigaard June 3, 2004, and February 6, 2013.
	3 IS, 8-isobutyl-12-ethyl, famesyl
	Molecular image,  BChlc_Mizoguchi_1996_molec.jpg: Mizoguchi, T., K. Matsuura, K. Shimada and Y. Koyama (1996). The structure of the aggregate form of bacteriochlorophyll c showing the Qy absorption above 740 nm: a 1H-NMR study. Chemical Physics Letters 260(1-2): 153-158.
	H-NMR spectroscopy and electronic absorption spectra.  S[I, E] BChl c F, i.e. bacteriochlorophyll c (3 ~S, 8-isobutyl-12-ethyl, farnesyl), was collected by HPLC from a mixture of bacteriochlorophyll c, which was extracted from the cells of Chlorobium limicola f. sp. Thiosulfatophilum. BChl c was dissolved into a 1 : 3 mixture of methylene chloride-d2 and carbon tetrachloride, and titrated in methanol-d4.
	BChl-c absorbance peaks at 456 and 746 nm.  Shoulder around 510 nm due to carotenoids.


Keywords:	bacteriochlorophyll c, carotenoids, Chlorobium tepidum, Chlorobium limicola f., green sulfur bacteria, absorbance spectrum, photosynthetic pigment, in vivo

Datafile name:	BChlc__invivo_Frigaard_2004_spec.csv

Misc:	Molecular structure
	BChlc_Mizoguchi_1996_molec.jpg